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Agents free formalin fixation for histological staining of tissue samples
Agents free formalin fixation for histological staining of tissue samples
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机译:试剂无福尔马林固定用于组织样品的组织学染色
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摘要
A fixative for tissue samples containing - at least one polyamine, which is selected from the group consisting of urotropin, dimetiloldihidroxietilen- urea, mono-, di-, tri-, tetra-, penta- or hexamethylolmelamine, tetramethylol-acetylene diurea, dimetilolpropilen- urea, acetoguanamine or 5,5-dimethyl hydantoin and mixtures thereof, and - at least one acidifying agent that is selected from the group of mono-, di- or free multivalent consisting of oxalic acid, fumaric acid, tartaric acid, maleic acid, succinic acid, hydrochloric acid, acetic acid, propionic acid, formic acid, mono- acid, di-, trichloroacetic, boric acid, phosphoric acid or a mixtures thereof, wherein the polyamine reacts with the protons released by the acid and in this case free form or aldehydes, and the molar ratio between the concentration of aldehyde per mole of amine to concentrated molar protons per mole of acid tion is in a range of 1: 0.7 to 1: 1.5.
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