MASS SPECTROMETRY-BASED METHODS FOR THE DETECTION OF CIRCULATING HISTONES H3 AND H2B IN PLASMA FROM SEPSIS OR SEPTIC SHOCK (SS) PATIENTS

摘要

The present invention proposes a novel method for the detection of circulating histones H3 and H2B in plasma from sepsis or septic shock (SS) patients, based on mass spectrometry-based methods, in particular based on multiple reaction monitoring targeted mass spectrometry (MRM-MS). Such methods allow quantification of histones using an internal standard and show strong specificity and sensitivity values.