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METHOD FOR PREPARING RETICULOCYTE SIMULATED PARTICLE AND PLATELET SIMULATED PARTICLE, AND QUALITY CONTROL MATERIAL
METHOD FOR PREPARING RETICULOCYTE SIMULATED PARTICLE AND PLATELET SIMULATED PARTICLE, AND QUALITY CONTROL MATERIAL
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机译:网状模拟颗粒和血小板模拟颗粒的制备方法及质量控制材料
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摘要
Provided are a method for preparing reticulocyte simulated particles and platelet simulated particles, and a quality control material. The method for preparing the reticulocyte simulated particles uses an N-hydroxysuccinimide-activated protein fluorescent dye to stain mammalian enucleated red blood cells with a volume of 60-120 fL and performs the fixed treatment to the enucleated red blood cells to prepare reticulocyte simulated particles. The platelet simulated particles are prepared using mammalian enucleated red blood cells with a volume of 2-25 fL, and the rest is the same as that for the reticulocyte simulated particles. The preparation method comprises: using an N-hydroxysuccinimide-activated protein fluorescent dye to stain different volumes of mammalian enucleated red blood cells, and obtaining reticulocyte simulated particles and platelet simulated particles respectively. The scatter diagram fluorescence and volume direction thereof are close to the scatter diagram distribution of reticulocytes, reticulated platelets, and platelets in fresh blood. The prepared simulated particles have good stability and do not interfere with the count classification of other cells and particles.
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