首页> 外国专利> PRIMERS AND PROBE FOR DETECTION OF MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS AND DETECTING METHOD FOR MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS USING THE SAME

PRIMERS AND PROBE FOR DETECTION OF MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS AND DETECTING METHOD FOR MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS USING THE SAME

机译:中东呼吸综合征冠状病毒检测的标杆和探针以及使用相同方法检测中东呼吸综合征冠状病毒的方法

摘要

The present invention relates to a primer and a probe for detection of Middle East respiratory syndrome coronavirus and to a method for detecting Middle East respiratory syndrome coronavirus using the same. Specifically, the primer and the probe comprising a specific nucleotide sequence targeting a nucleocapsid gene of the present invention have higher sensitivity to RT-PCR than previously used MERS real-time PCR kits targeting upE and ORF1a genes and primer and probe sets of a N2 gene to accurately detect MERS-CoV, and thus the primer and probe set of the present invention can be usefully used for detection and diagnosis of the MERS-CoV.
机译:用于检测中东呼吸综合征冠状病毒的引物和探针技术领域本发明涉及用于检测中东呼吸综合征冠状病毒的引物和探针,以及使用它们的检测中东呼吸综合征冠状病毒的方法。具体地,与针对upE和ORF1a基因以及N2基因的引物和探针组的先前使用的MERS实时PCR试剂盒相比,包含靶向本发明的核衣壳基因的特定核苷酸序列的引物和探针对RT-PCR具有更高的敏感性。为了准确地检测MERS-CoV,因此本发明的引物和探针组可用于检测和诊断MERS-CoV。

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