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PRIMERS AND PROBE FOR DETECTION OF MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS AND DETECTING METHOD FOR MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS USING THE SAME
PRIMERS AND PROBE FOR DETECTION OF MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS AND DETECTING METHOD FOR MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS USING THE SAME
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机译:中东呼吸综合征冠状病毒检测的标杆和探针以及使用相同方法检测中东呼吸综合征冠状病毒的方法
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摘要
The present invention relates to a primer and a probe for detection of Middle East respiratory syndrome coronavirus and to a method for detecting Middle East respiratory syndrome coronavirus using the same. Specifically, the primer and the probe comprising a specific nucleotide sequence targeting a nucleocapsid gene of the present invention have higher sensitivity to RT-PCR than previously used MERS real-time PCR kits targeting upE and ORF1a genes and primer and probe sets of a N2 gene to accurately detect MERS-CoV, and thus the primer and probe set of the present invention can be usefully used for detection and diagnosis of the MERS-CoV.
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