METHOD FOR CRYOPROTECTION OF FREE-FLOATING BRAIN SLICES FOR IMMUNOHISTOCHEMICAL RESEARCH

摘要

FIELD: pharmacology.;SUBSTANCE: invention is a method of cryoprotection of free-floating brain slices for immunohistochemical research, including brain slices fixation in a formaldehyde solution, placement in a solution based on phosphate-buffered saline NaCl, Na₂HPO₄ and NaH₂PO₄ with a cryoprotectant and storage at a reduced temperature, characterized in that glycerine in a volume ratio of 50:50 is added to the solution as a cryoprotecting agent.;EFFECT: invention allows to create a more economical method of brain slices cryoprotection and a less laborious process of its preparation due to a decrease in the number of components of the cryoprotecting solution.;2 dwg, 1 ex