METHOD FOR PRODUCING MYROTHECIUM VERBUCARIA VKM F-3851 FUNGUS LACCASES, TRANSFORMING PHENOLIC COMPOUNDS IN NEUTRAL-ALKALINE ENVIRONMENT CONDITIONS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology. Method for producing laccase of a bacteria of Myrothecium verrucaria VKM F-3851 strain provides submerged cultivation of Myrothecium verrucaria mineral environment VKM F-3851, containing as a natural carbon source and energy, at least one component selected from potato, rye, wheat, buckwheat, oats, barley, corn, peas, beans, as well as NH₄NO₃, KH₂PO₄, K₂HPO_4, MgSO₄x7H₂O in the given ratio of components. Strain is cultured with stirring for 2–10 days and at a temperature of 29 °C until the maximum activity of laccase in the culture liquid is obtained, followed by separation of the mycelium from the culture liquid.;EFFECT: invention makes it possible to obtain laccase, capable of transforming phenylpropanoids.;5 cl, 2 dwg, 13 ex, 2 tbl