METHOD FOR DETERMINING INHIBITOR OF TRYPSIN IN SOYBEANS AND THEIR DERIVATIVE PRODUCTS

摘要

FIELD: biochemistry; agriculture; food industry. ;SUBSTANCE: invention relates to biochemistry, agriculture and the food industry. Method for determining a trypsin inhibitor in soybeans and their processing products, including the selection and preparation of the analyzed sample, extraction of the soybean inhibitor into the solution, measurement and calculation of trypsin inhibitory activity, differs in that the extraction of the trypsin inhibitor in the test solution is carried out during the homogenization, centrifugation and filtration of the test sample for 10–15 minutes, to determine the activity of trypsin kinetic method, mix soy extract, reagent 1 (buffer pH 8.2) and a control solution of trypsin, incubate for 4 minutes, BAPNA substrate reagent is added, the trypsin inhibitory activity (TIA) is calculated by the formula: TIA = ((TI × 0.025) : 100) × 10000 : 4, where Ti is the amount of trypsin inhibitor, %, calculated by the formula: Ti = ((K – O) : K) × 100 %; K – activity of trypsin in the control sample, units/l; O – activity of trypsin in the test sample, units/l; 0.025 is the coefficient of transfer of the trypsin inhibitor from the percentages to the quantitative expression in mg; 100 – conversion factor from percent to mg; 10,000 – conversion factor from 1/10,000 to 1 g; 4 – incubation time of solution at 37 °C, min.;EFFECT: method for determining a trypsin inhibitor in soybeans and the products of their processing is proposed.;1 cl, 4 ex, 1 tbl