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METHOD OF DETECTION AND/OR IDENTIFICATION OF PROTOZOA OF GENUS BABESIA, PRIMER FOR USE IN THIS METHOD AND KIT FOR USE IN METHODS OF DIAGNOSIS OF SYMPTOMATIC AND ASYMPTOMATIC BABESIOSIS
METHOD OF DETECTION AND/OR IDENTIFICATION OF PROTOZOA OF GENUS BABESIA, PRIMER FOR USE IN THIS METHOD AND KIT FOR USE IN METHODS OF DIAGNOSIS OF SYMPTOMATIC AND ASYMPTOMATIC BABESIOSIS
The invention relates to a method of detection and/or identification of protozoa of the Babesia genus, including in particular B. microti, B. venatorum, B. canis and/or B. divergens, in samples containing nucleic acids, the method comprising the following steps: step of amplification of nucleic acids in single reaction, using primers: Primer 1 of sequence SEQ ID NO: 1 and/or Primer 2 of sequence SEQ ID NO:2 and Primer 3 of sequence SEQ ID NO:3; detection step; and optionally identification step. The invention also relates to a primer for use in a method of detection and/or identification of protozoa of the Babesia genus, use of these primers and a kit for use in methods of diagnosis of symptomatic and asymptomatic babesiosis, for detection and/or identification of protozoa of genus Babesia.
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机译:本发明涉及一种在含有核酸的样品中检测和/或鉴定巴贝斯氏菌属的原生动物的方法,所述原生动物尤其包括微小隐孢子虫,维纳托氏菌,犬双歧杆菌和/或分支杆菌。后续步骤:使用引物在单个反应中扩增核酸的步骤:序列SEQ ID NO:1的引物1和/或序列SEQ ID NO:2的引物2和序列SEQ ID NO:3的引物3;检测步骤;以及可选的识别步骤。本发明还涉及用于检测和/或鉴定巴贝氏菌属的原生动物的方法的引物,这些引物的用途和用于诊断和/或鉴定有症状和无症状巴贝病的方法的试剂盒。巴贝虫属的原生动物。
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