SECRETED PROTEIN ACIDIC AND RICH IN CYSTEINE (SPARC) PROTEIN SRM/MRM ASSAY

摘要

The present invention is related to a method for measuring the level of the Secreted Protein Acidic and Rich in Cysteine (SPARC) protein in a human biological sample of formalin fixed tissue, comprising detecting and quantifying the amount of a SPARC fragment peptide in a liquid, soluble, dilutable protein digest prepared from said human biological sample by heating and proteolysis, wherein said detecting and/or quantifying is carried out using mass spectrometry; and calculating the level of SPARC protein in said sample; wherein the SPARC fragment peptide is selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6; wherein said level is a relative level or an absolute level; and wherein the method further comprises selecting for the subject from which said biological sample was obtained a treatment based on the presence, absence, or amount of one or more SPARC fragment peptides or the level of SPARC protein.