CORTICAL INTERNEURONS AND OTHER NEURONAL CELLS PRODUCED BY DIRECTED DIFFERENTIATION OF PLURIPOTENT AND MULTIPOTENT CELLS

摘要

To provide cortical interneurons and other neuronal cells and in vitro methods for producing such cortical interneurons and other neuronal cells by directed differentiation of stem cells and neuronal progenitor cells.SOLUTION: The present disclosure relates to novel methods of in vitro differentiation of stem cells and neural progenitor cells to produce several types of neuronal cells and their precursor cells, including cortical interneurons, hypothalamic neurons and pre-optic cholinergic neurons. The present disclosure describes derivation of these cells via inhibiting SMAD and Wnt signaling pathways and activating SHH signaling pathways.SELECTED DRAWING: None