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Extraction method of herbacetin from Iwabenkei plant
Extraction method of herbacetin from Iwabenkei plant
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机译:岩本溪植物中除草素的提取方法
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摘要
The present invention discloses a method for extracting herbacetin from a plant belonging to the genus Solanum comprising the following steps. 1) The pulverized Iwabenkei medicinal material is extracted with an extraction solvent, and the extract is concentrated under reduced pressure to obtain an extract extract. 2) Extract the extract and further hydrolyze the aqueous layer after extraction. 3) Extract the acid-hydrolyzed solution with an organic solvent, mix the extracts, and concentrate under reduced pressure to obtain a herbacetin extract. 4) The herbacetin extract is treated with polyamide column chromatography, and the eluate containing herbacetin is collected and concentrated to dryness to obtain crude herbacetin. 5) Treat the crude herbacetin product with reverse phase silica gel column chromatography, collect the eluate containing herbacetin, concentrate to dryness and recrystallize to obtain the purified herbacetin product. The present invention produces herbacetin, the process is simple, the yield is high, and high-purity herbacetin can be produced.The purity of herbacetin reaches 90.0% to 99.9%, the specificity is diversified and the mass production It is suitable and provides a solid material base for research that explores pharmacological activity such as anti-oxidation of Iwabenkei, treatment of cardiovascular diseases, anti-tumor etc.
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