Method utilizing piggybac transposon and transposase to introduce mutation into target DNA in a plant cell

摘要

A method for producing a plant cell comprising a mutation introduced in a target DNA comprises:a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA;a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; anda step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.