METHOD TO PRODUCE PROTEIN IN ASPERGILLUS NIGER`S SLEEPING SPORES USING SSRNA

摘要

The present invention discloses A METHOD TO PRODUCE PROTEIN IN ASPERGILLUS NIGER'S SLEEPING SPORES USING single stranded RNA. The method includes three steps of culture of Aspergillus niger and collection of spores, pretreatment of Aspergillus niger spores, and electroporation of Aspergillus niger spores by using HDEN method. In the present invention, non-germinated spores are used as a starting material for introduction of exogenous molecules. The exogenous protein coding single stranded RNA is introduced into the resting spores of Aspergillus niger by employing the HDEN electrotransformation technique to express protein. The method of this invention is simple and fast, the effect is excellent, and the transformation rate reaches more than 90%.