Method for direct transformation of exogenous DNA into resting spores of Aspergillus niger independent of mediators

摘要

The present invention discloses a method for direct transformation of exogenous DNA into resting spores of Aspergillus niger independent of mediators. The method includes three steps of culture of Aspergillus niger and collection of spores, pretreatment of Aspergillus niger spores, and electroporation of Aspergillus niger spores by using HDEN method, to obtain Aspergillus niger spores with introduction of plasmids to be transformed. In the present invention, non-germinated spores are used as a starting material for introduction of an exogenous molecule, and exogenous DNA is introduced into the resting spores of Aspergillus niger by employing the HDEN electrotransformation technique, whereby the complex step of spore germination is omitted, and steps of protoplast preparation or Agrobacterium-mediated transformation in conventional methods etc. are omitted. Moreover, the transformation efficiency is high, and at least an effect of no less than 6000 positive transformants per transformation reaction system can be achieved.