Messenger RNA based expression of opsins and reporter proteins for electrophysiologic characterization of in vitro neurons and cardiomyocytes

摘要

Synthetic oligonucleotides are used to express opsins in cells, such as neurons and cardiomyocytes, for rapid induction of light-responsive electrophysiological behavior. Such induction can change the electrical properties of the cell. MEA analysis and use of voltage indicator proteins are described. Methods of testing drugs for their effect on electrical properties of the cell are described, along with methods for screening drugs that have an effect on a cell's electrical properties.