METHOD FOR MANUFACTURING BASIC CULTURE MEDIA FOR MESENCHYMAL STEM CELL, BASIC CULTURE MEDIA FOR MESENCHYMAL STEM CELL AND CELL THERAPHY PRODUCTS CULTURED AND DIFFERENCIATED USING THE SAME

摘要

PURPOSE: minimal medium is that mesenchymal stem cell is provided as the proliferation rate of enhancing stem cell and to shorten culture time. ;CONSTITUTION: minimal medium is mescenchymal stem cell, the basic media ABM-M (advance) of mescenchymal stem cell, basic DMEM high glucose mediums and has contained other ingredient. Contain L-Alanine 9mg/L, zinc chloride L-Asparagine 50mg/L, L-Aspartic acid 20mg/L, LGlutamic acid 20mg/L, L-Hydroxy-L-proline 20mg/L, L-Proline 34. in the DMEM high glucose mediums5mg/L, cupric sulfate pentahydrate 0.0025mg/L, ferrous sulfate heptahydrate 0.834mg/L, disodium hydrogen phosphate zinc chloride 142.04mg/L, zinc sulfate heptahydrate 0.863mg/L, D-Biotin 0.2mg/L, P-Aminobenzoic acid (PABA) 1mg/L, vitamin B12 1.36mg/L, Hypoxanithine 4.The 1mg/L that 08mg/L, L-Glutathione are reduced, linoleic acid 0.084mg/L, Putrescine+2HCL 0.161mg/L, dithio-octanoic acid 0.21mg/L, Thymindine 0.73mg/L. ;The 2012 of copyright KIPO submissions