Biological antifugal liquid preparation with microorganism pythium oligandrum and method of production

摘要

The liquid biological antifungal product containing the Pythium oligandrum microorganism containing a stabilized suspension of the Pythium oligandrum microorganism contains 0.05 to 10.0 % weight culturing biomass of the Pythium oligandrum microorganism with content of cultivation medium, cell forms of this microorganism and substances produced by this microorganism and 90.0 to 99.95 % weight stabilizer, whereby the pre-determined number of dormant oospores in 1 ml of this liquid biological antifungal product is, following normal standardization, between 1 x 103 and 2 x 107. The liquid biological antifungal product containing the Pythium oligandrum microorganism containing a stabilized suspension of the Pythium oligandrum microorganism contains 0.05 to 10.0 % weight culturing biomass of the Pythium oligandrum microorganism with content of cultivation medium, cell forms of this microorganism and substances produced by this microorganism; and 79.77 to 99.95 % weight stabilizer, and the remainder, up to 100 % weight, a minimum of one modify ing/application substance from a group including filling, aroma and vitamin; whereby the pre-determined number of dormant oospores in 1 ml of this liquid biological antifungal product is, following normal standardization, between 2.5 x 104 and 1.0 x 106. The Pythium oligandrum microorganism is the Pythium oligandrum Dreschler ATTC 38472 strain, which was deposited in the Czech Collection of Microorganisms (CCM) at Masaryk University in Brno under the appellation of Pythium oligandrum M1. The stabilizer may be water, a salt solution, oil or a concentrated solution of osmolytes. The method of production of this liquid biological antifungal product is claimed.