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Direct capture using large bead chromatography media.
Direct capture using large bead chromatography media.
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机译:使用大珠层析介质直接捕获。
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摘要
Continuous process in which a subset of a number of mutually identical columns, are connected in series. The process liquid, e.g. crude cell culture harvest, is supplied to the most upstream column of the subset, flows successively through the in series connected columns and leaves the subset through the most downstream and flows e.g. into the downstream collection vessel. As soon as the packed bed of the most upstream column is become saturated with product, this column is disconnected from the subset and removed from the series connection and a replacement, identical, column is added such that it is connected in series downstream from the most downstream column of the subset and this process is repeated.
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