CLARIFICATION METHOD FOR OBSERVATION OF THREE-DIMENSIONAL CELL CULTURES MARKED WITH PROPODIUM IODIDE THROUGH CONFOCAL MICROSCOPY

摘要

THIS INVENTION CONCERNS THE DEVELOPMENT AND APPLICATION OF AN OPTICAL CLEARING METHOD FOR OBSERVATION OF SAMPLES THROUGH THE USE OF CONFOCAL MICROSCOPY, IN PARTICULAR THREE-DIMENSIONAL OR PREAMOID SPHERIOUS CELL CULTURES. THE METHOD INCLUDES IMMERSION OF SPHEROIDS IN FORMAMIDE SOLUTIONS IN ORDER TO HOMOGENIZE THE REFRACTION INDEX. IN A LARGER AMPLITUDE.