WELL PLATE-BASED PERFUSION CULTURE MODEL OF ENDOSTEAL-, EXTRACELLULAR MATRIX (ECM)- AND ENDOTHELIAL- MYELOMA INTERACTIONS AND METHODS FOR TESTING PERSONALIZED THERAPEUTICS FOR MULTIPLE MYELOMA

摘要

The described invention provides a well plate-based perfusion culture model of endosteal-, extracellular matrix (ECM)- and endothelial- myeloma interactions and patient-specific methods for selecting treatment for and assessing drug resistance of multiple myeloma (MM). The described methods utilize an ex vivo three dimensional endosteal microenvironment effective to recapitulate spatial and temporal characteristics of a multiple myeloma cancer niche and to maintain viability of multiple myeloma cells (MMCs) obtained from a patient suffering from MM.