DEPRESSIVE OR EPILEPTIC ANIMAL MODEL, ESTABLISHMENT METHOD THEREFOR, AND METHOD FOR SCREENING CANDIDATE DRUG FOR THERAPY OF DEPRESSIVE DISORDER OR EPILEPSY USING SAME

摘要

The present invention relates to a depressive or epileptic animal model and an establishment method therefor using CRISPR/Cas9 molecular scissors technology and further to a method for screening a candidate drug for therapy of depressive disorder or epilepsy using the animal model. Lacking most of the Kcnk1 gene, in contrast to the mice from which the Kcnk1 gene is partially deleted by classical genetic recombination techniques, Kcnk1 gene-knockout mice established using CRISPR/Cas9 molecular scissors according to the present invention show intrinsic symptoms of depressive disorder and/or epilepsy with the onset of depressive disorder and/or epilepsy therein and are expected to be useful as an animal model for research on the progression and pathological mechanism of depressive disorder and/or epilepsy. In addition, it is expected that the Kcnk1-knockout mice according to the present invention are effectively used for screening candidate drugs for prophylaxis or therapy of depressive disorder and/or epilepsy.