首页>
外国专利>
NON-HUMAN TRANSGENIC MAMMAL, PROTEIN CRUDE EXTRACT SEPARATED FROM CONNECTIVE TISSUE OF NON-HUMAN TRANSGENIC MAMMAL, METHOD FOR PROTEIN CRUDE EXTRACT, AND USES OF PROTEIN CRUDE EXTRACT
NON-HUMAN TRANSGENIC MAMMAL, PROTEIN CRUDE EXTRACT SEPARATED FROM CONNECTIVE TISSUE OF NON-HUMAN TRANSGENIC MAMMAL, METHOD FOR PROTEIN CRUDE EXTRACT, AND USES OF PROTEIN CRUDE EXTRACT
A non-human transgenic mammal, a protein crude extract separated from a connective tissue of the non-human transgenic mammal, a method for the protein crude extract, and uses of the protein crude extract. The method comprises: transferring a deoxyribonucleic acid sequence construct (DNA construct) containing SEQ ID NO:4 or SEQ ID NO:5, into a rat embryo, transplanting the rat embryo into a female rat of the same species to develop into a mature rat, and separating a protein crude extract containing a target amino acid sequence from a connective tissue of the mature rat, the target amino acid sequence being: (i) an amino acid sequence of SEQ ID NO:1 and an amino acid sequence of SEQ ID NO:60; or (ii) an amino acid sequence of SEQ ID NO:2 and an amino acid sequence of SEQ ID NO:61; or (iii) the amino acid sequence of SEQ ID NO:1, the amino acid sequence of SEQ ID NO:2, and the amino acid sequence of SEQ ID NO:60 or the amino acid sequence of SEQ ID NO:61.
展开▼
机译：非人类转基因哺乳动物，从非人类转基因哺乳动物的结缔组织中分离的蛋白质粗提物，蛋白质粗提物的方法和蛋白质粗提物的用途。该方法包括：将含有SEQ ID NO：4或SEQ ID NO：5的脱氧核糖核酸序列构建体（DNA构建体）转移到大鼠胚胎中，将该大鼠胚胎移植到相同物种的雌性大鼠中以发育成成熟大鼠。从成熟大鼠的结缔组织中分离含有靶氨基酸序列的蛋白质粗提物，所述靶氨基酸序列为：（i）SEQ ID NO：1的氨基酸序列和SEQ ID NO：1的氨基酸序列NO：60; （ii）SEQ ID NO：2的氨基酸序列和SEQ ID NO：61的氨基酸序列;或（iii）SEQ ID NO：1的氨基酸序列，SEQ ID NO：2的氨基酸序列，SEQ ID NO：60的氨基酸序列或SEQ ID NO：61的氨基酸序列。
展开▼
