USE OF MAIZE GENE ZMABCG20 IN REGULATING CROP MALE FERTILITY AND DNA MOLECULAR MARKERS ASSOCIATED WITH MAIZE MALE FERTILITY AND USE THEREOF

摘要

Provided is a use of maize gene ZmABCG20 in regulating male fertility of crops. The genomic DNA sequence of ZmABCG20 in maize variety B73 is shown by SEQ ID NO: 1, and the encoded protein sequence is shown in SEQ ID NO: 3. Also provided is a mutant zmabcg20-1 of the gene ZmABCG20 and use thereof, and the mutated gene sequence is shown in SEQ ID NO: 7. Also provided is a molecular marker identification method of the mutated gene. Also provided is a DNA molecular marker associated with male fertility in maize, the DNA molecule marker being located at bases 326-329 after the start codon of the maize gene ZmABCG20 nucleic acid sequence, and the sequence is TGCA, and the 4 base deleted maize line exhibits recessive male sterility. The DNA molecular marker of the present invention can be used to identify or breed male sterile maize germplasm resources and the like.