METHOD FOR DETECTING OLEFIN COMPONENTS IN ORANGE JUICE THROUGH ONLINE-SOLID-PHASE MICRO-EXTRACTION AND COMPREHENSIVE TWO-DIMENSIONAL GAS CHROMATOGRAPHY/QUADRUPOLE MASS SPECTROMETER

摘要

#$%^&*AU2019100394A420190516.pdf#####ABSTRACT The present invention discloses a method for identifying and analyzing olefin components in orange juice through online-solid-phase micro-extraction (online-SPME) and comprehensive two-dimensional gas chromatography/quadrupole mass spectrometer (comprehensive 2DGC/qMS). The method comprises the steps: enriching olefin components in an orange juice sample through online-SPME; detecting through comprehensive 2DGC/qMS; conducting qualitative analysis on a determinand through a combination of a retention time index (LRI) and NIST spectral library; and accurately quantitatively analyzing chromatographic overlapping peaks by a nodal convolution technique for the components which cannot be separated by the comprehensive 2DGC. The comprehensive 2DGC adopts two chromatographic columns with different polarities for conducting orthogonal separation on the olefin components, and qMS is conducted under the effect of a modem for completing detection. Qualitative analysis is conducted through the combination of the LRI and the NIST spectral library, and the chromatographic overlapping peaks are accurately quantitatively analyzed by the nodal convolution technique. The method of the present invention has the characteristics of high throughput, high sensitivity and high accuracy, and solves the problems of difficult analysis and separation and difficult qualitative analysis for the olefin components in the orange juice.