METHOD FOR INDUCING HUMAN SPINAL MOTOR NEURON PROGENITORS TO DIFFERENTIATE INTO SPINAL MOTOR NEURONS

摘要

Provided is a method for inducing human spinal motor neuron progenitors to differentiate into spinal motor neurons, comprising the following steps: 1) culture of the spinal motor neuron progenitors: digesting the spinal motor neuron progenitors into single cells, inoculating the single cells into a coated petri dish for adherent culture with a spinal motor neuron progenitor culture medium, and culturing in an incubator at 37°C, 5% CO₂ and saturated humidity; and 2) induction of the spinal motor neurons: culturing the spinal motor neuron progenitors obtained from step 1) in a basic complete medium, adding different space-time-specific signaling pathway modulating small molecules and/or growth factors at different time points to induce differentiation, culturing for 3-30 days in an incubator at 37°C, 5% CO₂ and saturated humidity, changing the medium every other day.