PERFUSION LOOP ASSEMBLY FOR AN EX-VIVO LIVER PERFUSION AND A METHOD FOR EX-VIVO LIVER PERFUSION

摘要

The invention refers to a perfusion loop assembly for ex vivo liver (100) perfusion, comprising at least one pump (101) for providing a fluid flow of a perfusion fluid through a line that branches into a first branch line (110) and a second branch line (120) downstream of the pump; the first branch line being configured to provide a first portion of the perfusion fluid to the hepatic artery (111) and being coupled with at least one first gas exchanger (112) and comprising at least one flow rate and/or pressure sensor (114, 113), the second branch line being configured to provide a second portion of the perfusion fluid to the portal vein (121) and comprising at least one first valve (122) for controlling the flow of the perfusion fluid into the portal vein, the second branch line further comprising at least one flow rate and/or pressure sensor (124, 123); a liver chamber assembly (130); and an outlet line connecting the liver chamber assembly and the pump. Also disclosed are a method for stimulating bile production, and a spectroscopic method for measuring cellular signal molecules to characterise ischemic injury.