THIOLATED CHITOSAN DERIVATIVE, CHITOSAN HYDROGEL, AND PREPARATION METHODS THEREFOR AND APPLICATIONS THEREOF

摘要

The present disclosure relates to a thiolated chitosan derivative, a chitosan hydrogel, a double crosslinked chitosan hydrogel, and preparation methods therefor and applications thereof. Alpha-beta unsaturated structures are respectively grafted onto chitosan molecule chains, thiol groups are grafted onto the chitosan molecule chains, and a chitosan hydrogel is obtained by means of a Michael addition reaction. A hydrogel obtained by reacting an alpha-beta unsaturated acylated chitosan with a thiolated chitosan is soaked in an ethanol solution to obtain a double crosslinked chitosan hydrogel. Chitosan is used as a raw material, and a sulfo compound having both sulfo groups and carboxyl groups is used as a modifying agent. Under the effect of a carboxyl group activating agent, and with the strong electron withdrawing effect of the sulfo groups, the sulfo groups are successfully introduced to the amino groups and the primary hydroxyl groups of the chitosan, and the sulfo groups are then converted into thiol groups via reduction to obtain the thiolated chitosan derivative of the present disclosure. The hydrogel of the present disclosure has a rapid curing speed, high mechanical strength, good biocompatibility, good stability in culture media, and a wide application range.