N Akabane viruses blocking ELISA using monoclonal antibodies against recombinant N protein

摘要

The present invention relates to a hybridoma cell line, KCTC18469P, which is characterized by producing a monoclonal antibody 5B56 against a recombinant N protein of Akabane virus; a monoclonal antibody 5B56 produced from the cell line; a detection kit comprising the monoclonal antibody; and a method for detecting infection with Akabane virus, which measures the level of a detection marker prepared by reacting the monoclonal antibody with an examination target sample through antigen-antibody binding. The monoclonal antibody against the recombinant N protein of Akabane virus used in the method for detecting Akabane virus infections according to the present invention has excellent binding capacity with respect to a recombinant N protein antigen; may be continuously produced; and has excellent detection sensitivity and specificity. Accordingly, the monoclonal antibody can detect virus antibodies more rapidly in a larger quantity than conventional methods using serum tests, and can be used conveniently in monitoring a disease in animals, and thus, can be beneficially used for an effective detection method and detection kit for Akabane virus. Further, by using domestically isolated Akabane virus antigens in production of the detection kit, antibodies infected with epidemic viruses in Korea can be detected with higher sensitivity.