- - Knock-in vector for expression of bovine lactoferrin and knock-in method in the bovine embryo using the same

摘要

The present invention relates to a knock-in vector for bovine lactoferrin expression and a knock-in method in a bovine embryo using the knock-in vector. More particularly, the present invention provides a bovine beta-casein genomic DNA knock-in vector, comprising: a 5′-terminal fragment comprising a nucleic acid sequence represented by SEQ ID NO: 1 and comprising a promoter of a sub-beta-casein genomic DNA and a part of exon 7, as a 5′-arm; a gene encoding a therapeutic protein; and an intron portion between exon 7 and 8 of the bovine beta-casein genomic DNA, comprising the nucleic acid sequence shown in SEQ ID NO: 4, as a 3′-arm.