Fermentation method to improve recombinant human collagen production level

摘要

The present invention provides a fermentation method for improving the production level of recombinant human collagen. First, the Pichia pastoris isolate is inoculated into a sterilized fermentation medium, fermented for 14 to 18 hours, supplemented with methanol to induce induction, and sodium pyruvate is added to the fermentation medium, wherein sodium pyruvate The addition amount is 0.01 to 10 g / L.