Culture method of human salivary gland cells

摘要

The object of the present invention is to increase the number of submerged cultures of human salivary gland cells and to maintain undifferentiated state and high proliferation possibility during culturing. (a) obtaining human salivary epithelial progenitor cells from the recipient organism; (b) Cells were transferred to PCT epidermal keratinocyte culture medium, cultured in culture flask, 5% CO ₂ was added, and the medium was changed every 2 to 4 days until a monolayer was formed, Assuring adhesion; (c) subculturing the cells at a dilution ratio of 1: 3 to 1: 5, except that the EDTA trypsin solution is used to separate the cells from the culture flask surface and then transferred to a new culture flask; ; (d) further performing cell culture as defined in step (b), wherein the intermediate medium is exchanged every 2 to 4 days, and a single layer as defined in step (c) Culturing human salivary epithelial progenitor cells containing submerged culture at a maximum dilution ratio of 1: 2 to 1: 3 until formation, wherein the culture medium first exchanged after each subculture should be performed within 8 to 24 hours] Method is provided.