DECREASING LACTATE LEVEL AND INCREASING POLYPEPTIDE PRODUCTION BY DOWNREGULATING LDH AND PDHK EXPRESSION

摘要

The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the present invention relates to a method of culturing cells expressing siRNA specific for a) small interfering RNA (siRNA) specific for lactate dehydrogenase (LDH) and b) pyruvate dehydrogenase kinase (PDHK) The method comprising the steps of: In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for LDH and a siRNA specific for PDHK.