CD20 TRANSGENIC AVIAN PRODUCING IMPROVED MONOCLONAL ANTIBODIES SPECIFIC TO HUMAN CD20 AND A METHOD FOR PRODUCING THE SAME

摘要

The present invention relates to a transgenic bird producing an efficacy-improved human CD20-specific monoclonal antibody and a preparation method therefor. The preparation method for a transgenic bird according to the present invention can prepare a transgenic subject producing an efficacy-improved human CD20 specific monoclonal antibody at high yield and thus can be suggested as a novel model suitable for production of a biobetter. Glycosylation pattern analysis resulted in the finding that the human CD20-specific monoclonal antibody produced in the transgenic bird according to the present invention is in an afucosylated state and thus shows higher complement dependent cytotoxicity (CDC) than commercial CD20 monoclonal antibodies and about 8- to 16-fold higher efficiency in antibody dependent cell-mediated cytotoxicity (ADCC) than a control drug, so that the method is expected to be widely used as an economically feasible and stable antibody preparation method.