PCR DETECTING METHOD BARLEY YELLOW MOSAIC VIRUS FROM SOIL USING NESTED PCR

摘要

The present invention relates to a method for detecting Barley yellow mosaic virus (BaYMV) in soil using nestled PCR, which comprises a first step of isolating RNA from a soil sample surrounding barley cultivation; A second step of synthesizing cDNA using a reverse transcriptase using the RNA isolated in the first step as a template; A third step of performing first PCR using the cDNA synthesized in the second step as a template and primer combinations of SEQ ID NO: 1 and SEQ ID NO: 2; A fourth step of performing a secondary PCR using the primer combination of SEQ ID NO: 3 and SEQ ID NO: 4 using the first PCR product as a template; And a fifth step of identifying a barley-mosaic virus (BaYMV) gene from the secondary PCR product. The method of detecting barley-borne mosaic virus (BaYMV) in soil using nested PCR of the present invention is an effective detection technique with a high specificity and sensitivity, compared with the conventional method. In this method, microorganisms parasitic to the microorganism or the culture density are extremely It can be used for the diagnosis of low egg detection pathogens and it can be used as a useful tool to accurately diagnose the presence of soil infectious microorganisms in quarantine field.