METHOD OF IDENTIFICATION OF MYCOBACTERIA OF TUBERCULOSIS OF THE BEIJING GENOTYPE B0 CLUSTER IN REAL TIME FORMAT

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Task is achieved by identifying the Mycobacterium-specific tuberculosis of the Beijing B0-type genotype of a DNA region (insertion of the element IS6110 in the Rv2664-Rv2665 genome locus), amplified by PCR in real time. Analysis of the results is carried out by identifying the PCR product in real time using a specific fluorescent probe. FAM channel records the accumulation of the product of amplification of a DNA fragment specific for the Beijing B0 cluster through the channel of a HEX DNA fragment specific for the strain of any other M. tuberculosis genotype. When the Beijing B0 cluster is in the DNA sample, between 15–35 cycles of PCR, the fluorescence signal grows exponentially through the FAM detection channel and there is no fluorescence signal through the control HEX detection channel.EFFECT: method for the rapid and reliable detection of Mycobacterium tuberculosis of the Beijing B0 cluster genotype is proposed.1 cl, 3 dwg, 2 ex