METHOD FOR DETERMINING BACTERIAL ENDOTOXIN IN BIOLOGICAL FLUIDS

摘要

FIELD: clinical laboratory diagnostics.;SUBSTANCE: invention relates to clinical laboratory diagnostics and represents a method for determining bacterial endotoxin (BE) in blood plasma and urine, characterized by that preliminary preparation of blood plasma samples involves dilution of blood plasma samples with a physiological solution in 10–100 times and their heat treatment at 45–85 °C for 30–60 minutes, and preliminary preparation of urine samples involves urine extraction using methylene chloride or 1,2-dichloroethane; after which the reaction of the samples with the LAL reagent is carried out in the following sequence: 100 mcl of treated samples containing BE are poured into sterile test tubes, and there added is 100 mcl of LAL reagent, the tubes are thermostated for 1 hour at 37 °C to terminate the reaction, 50 mcl of 50 % acetic acid is added to each tube, followed by spectrophotometric determination of BE concentration in samples, including measurement of BE concentration as to optical density of sample after LAL-test at wavelength λ=388 nm.;EFFECT: invention provides reducing the number of false-positive results and increasing sensitivity when determining BE.;1 cl, 2 tbl, 6 ex