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ACCURATE, RAPID AND CONVENIENT SINGLE-STEP DISEASE DIAGNOSTIC METHOD USING SELF-AMPLIFICATION PRINCIPLE OF DETECTION SIGNAL
ACCURATE, RAPID AND CONVENIENT SINGLE-STEP DISEASE DIAGNOSTIC METHOD USING SELF-AMPLIFICATION PRINCIPLE OF DETECTION SIGNAL
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机译:使用检测信号自放大原理的准确,快速,便捷的单级病诊断方法
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摘要
The present invention relates to a method of accurately, rapidly, and conveniently detecting a disease marker by linking immunodiagnostics and Au particle formation at the same time and, accordingly, realizing self-amplification of a detection signal and a kit therefor, more particularly, to a method of detecting a disease-specific marker using self-amplification of a detection signal and a kit therefor, the method including (a) a step of simultaneously inducing an antigen-antibody immune response and an Au particle formation reaction by reduction of Au ions in an assay solution prepared by, to a pre-assay solution in which all of an antibody or antigen for detection of a disease-specific marker, free Au ions, and adsorbed Au ions are present, adding a sample, which contains a disease-specific antigen or antibody binding specifically to the antibody or the antigen, and a reducing agent; and (b) a step of confirming the presence or absence of a disease-specific marker by a chromogenic reaction through the Au particle formation. The method according to the present invention can diagnose a disease in a single step without any processes such as immobilization and washing processes that are generally accompanied in existing diagnosis methods such as ELISA; can short the time of a diagnostic test, which takes several hours or even several days, to about 10 minutes, thus being effectively used for diagnosis of diseases, in particular, of patients in an emergency; and can be confirmed by the naked eye without a separate analyzer, thus minimizing detection costs.
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