METHOD FOR STATISTICALLY DETERMINING A QUANTIFICATION OF OLD AND NEW RNA

摘要

The present invention provides a method for determining a quantification of old and new RNA for a gene, the method comprising:- obtaining reads from RNA fragments of a sample, wherein the RNA fragments comprise nucleotide substitutions caused by a metabolic marker,- determining statistics of nucleotide mismatches in the reads,- determining, based on the statistics of nucleotide mismatches, an error rate indicating a rate of nucleotide mismatches in old RNA and a conversion rate indicating a rate of nucleotide mismatches in new RNA, and- determining the quantification of old and newly synthesized RNA for the gene based on the error rate, the conversion rate and the statistics for the gene.