HEPATOCYTE CULTURE DEVICE ENHANCING ACCUMULATION AND EXCRETION OF HEPATIC METABOLITE IN AND INTO BILE CANALICULUS-LIKE STRUCTURE, AND METHOD FOR EVALUATING CANDIDATE COMPOUND SENSITIVE TO EXCRETION INTO BILE OR BLOOD AND HEPATIC METABOLITE OF THE CANDIDATE COMPOUND USING THE HEPATOCYTE CULTURE DEVICE

摘要

PROBLEM TO BE SOLVED: To provide a hepatocyte culture device enhancing accumulation and excretion of hepatic metabolite into and in bile canaliculus-like structure, and to provide a method for evaluating candidate compound sensitive to excretion into bile or blood and hepatic metabolite of the candidate compound using the hepatocyte culture device.;SOLUTION: An evaluation device is provided that comprises: a first cell culture containing a plurality of hepatocytes; a portable culture vessel in which a bile canaliculus-like structure can be established between the hepatocytes in the first cell culture, and which has, on the outer surface thereof, a membrane through which a physiologically active substance can pass; and a second cell culture which can increase the excretion activity of a hepatic metabolite in the first cell culture, the first cell culture cultured in the portable culture vessel being placed on the second cell culture and then the first cell culture and the second cell culture are co-cultured in the culture vessel to enhance the accumulation and excretion of a hepatic metabolite into a bile canaliculus-like structure.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2020,JPO&INPIT