Induction of neural progenitor cells, oligodendrocyte progenitor cells, and oligodendrocytes by stem cell differentiation using landmark transcription factors

摘要

A novel method for inducing human iPSC-derived neural progenitor cells, oligodendrocyte progenitor cells, and oligodendrocytes with unprecedented efficiency and functionality. The core of the present invention is a previously unknown mode at multiple key differentiation determinants along the pathway from pluripotency to ectoderm, neuroectoderm, from these to NPCs, from OPCs to oligodendrocytes. Is the use of transcription factors discovered experimentally. The present disclosure presents a specific combination and range of cell densities, reagents for the differentiation cascade of pluripotent stem cells into neural progenitor cells, oligodendrocyte progenitor cells, oligodendrocytes by a dynamically controlled cell growth process. Concerning induction and / or orientation utilizing concentrations, as well as specific combinations of mRNA.