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Methods for Measuring Intermolecular and / or Intramolecular Interactions
Methods for Measuring Intermolecular and / or Intramolecular Interactions
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机译:测量分子间和/或分子内相互作用的方法
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摘要
The following steps: a) Providing a sample comprising labeled particles and a ligand in a solution (where the labeled particles are dissolved or dispersed in the solution or immobilized on a solid support). B) The step of exciting the labeled particles with fluorescence and detecting the fluorescence of the excited particles at a predetermined temperature; c) The step of repeating steps (a) and (b) a plurality of times at different concentrations of the ligand in the solution; And d) for measuring the interaction between the labeled particle and the ligand, comprising the step of determining the interaction between the labeled particle and the ligand based on the ligand concentration dependent change of the fluorescence of the labeled particle. Method (where the labeled particles are labeled with one or more dyes).
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