Quantitative phase image generation method, quantitative phase image generator and program

摘要

The quantitative phase image generation method is a quantitative phase image generation method using a microscope, in which an object is irradiated with illumination light and an objective lens is located at a plurality of positions separated from each other along the optical axis of the objective lens by Δz. To detect the light from the object by arranging the focal point of, to generate the light intensity distribution data corresponding to each of multiple positions based on the detected light, and based on the light intensity distribution data, The interval Δz is set based on the setting information of the microscope, including generating a quantitative phase image.