Direct effect of extracted BMP on Mesenchymal Stem Cell Differentiation for use in a Culture Medium, Diagnostic Assay, and Adjunct or Stand-Alone Pharmaceutical Treatment

摘要

The present invention comprises a culture medium composition for the proliferation and differentiation of mesenchymal stem cells into osteoblasts, the composition comprising a bone morphogenic protein (BMP) complex with TGF-f3, bFGF, VEGF, and IGF, bound to collagen type 1 dissolved into a mesenchymal stem cell culture medium. Methods of use of the composition and/or the BMP complex comprise: performing a diagnostic assay detecting a level of bone morphogenetic proteins in a patient serum level; determining if the BMP level is below a threshold level; and when the BMP level is below the threshold level, administering a composition comprising a BMP complex, with or without a pharmaceutical, such as a drug for treating and/or preventing osteoporosis. The present invention comprises performing an in vivo lavage and bone assay; and administering the BMP complex composition to a patient in need thereof.