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METHOD FOR RAPIDLY AND EFFICIENTLY CREATING DIRECTED GENE MUTATED NON-TRANSGENIC PLANTS AND ITS APPLICATIONS
METHOD FOR RAPIDLY AND EFFICIENTLY CREATING DIRECTED GENE MUTATED NON-TRANSGENIC PLANTS AND ITS APPLICATIONS
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机译:快速有效地培育基因突变的非转基因植物的方法及其应用
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摘要
The invention relates to a plant genetic engineering field, and more particularly to a method for creating directed gene mutated non-transgenic plants. The method including performing a transgenic method onto directed gene mutated plants by introducing exogenous nucleic acid molecules; wherein the transgenic method includes introducing constructs into the directed gene mutated plants, each of the constructs contains a first nucleic acid molecule and a second nucleic acid molecule, wherein the first nucleic acid molecule serves as a gene editing element, and the second nucleic acid molecule serves as a lethal or stop development element, and can be used in a plant gene editing system such as CRISPR/CAS9. It can actively and automatically eliminate plant transgenic fragments, leaving enough time for gene editing elements to perform directed gene editing before removing transgenic fragments, providing a simple and effective method for gene editing without transgenic plants.
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