METHOD FOR INCREASING ACCURACY OF ANALYSIS BY REMOVING PRIMER SEQUENCE IN AMPLICON-BASED NEXT-GENERATION SEQUENCING

摘要

The present invention relates to a method for increasing the efficiency of read data analysis by removing primer sequence information present in a read obtained through next-generation sequencing (NGS) and, more specifically, to a method for matching information of a read and a designed primer to various reference values in several steps so as to determine primer sequence information within a read, and then precisely removing only a primer sequence so as to increase the efficiency of read data analysis. The method for increasing the efficiency of read data analysis in a primer removal-based NGS, according to the present invention, has a rapid data analysis speed and can precisely remove only a primer sequence, thereby being useful for increasing the efficiency and accuracy of read data analysis.