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Single Nucleotide Polymorphism (SNP) Markers for Phaseolus Vulgaris L. and Methods of Use Thereof In Selection Efficiency with Breeding Strategies
Single Nucleotide Polymorphism (SNP) Markers for Phaseolus Vulgaris L. and Methods of Use Thereof In Selection Efficiency with Breeding Strategies
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机译:菜豆的单核苷酸多态性(SNP)标记及其选择方法及选育策略
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摘要
The invention provides processes for marker assisted selection of common beans expressing volatile compounds that provide flavor traits associated with single nucleotide polymorphisms (SNPs) and/or sequences flanking SNPs, as well as allele-specific oligo sequence primers configured to anneal to related SNPs and to report the presence or absence of SNPs with fluorescent signals using a PCR assay, a KASP assay (i.e., modified PCR assay), or other molecular marker assay, e.g., SSR, capable of identifying the presence or absence of SNPs and/or portions of flanking sequences of the SNPs, all of which enhances selection efficiency in common bean breeding strategies.
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