SECOND GENERATION ETHANOL PRODUCTION FROM COCONUT LEAF BIOCONVERSION AS LIGNOCELLULOSIC BIOMASS

摘要

the present invention aims to increase the production of ethanol from lignocellulosic residues of the dwarf coconut leaf through enzymatic hydrolysis and fermentation, favoring the reduction of agricultural waste improperly discarded. therefore, the waste is reused as a raw material for the production of second generation ethanol. The process of producing ethanol from the dwarf coconut tree consists of the following steps: cutting the coconut tree leaf by separating the leaflets from the stem, cleaning with 100 ppm hypochlorite solution, drying in an oven at 45 ° c and grinding in a knife mill for increase the contact surface, chemical characterization, pretreatment with diluted sulfuric acid at a concentration of 2%, enzymatic hydrolysis with the cellic ctec2 enzyme complex (novozymes) and supplementation with cellic htec (novozymes), alcoholic fermentation with yeasts kluyveromices marxianus, ending with the determination of the alcohol content by high performance liquid chromatography. the amount of 2g ethanol produced was 5.66 g.l-1 for the leaflets and 6.42 g.l-1 for the rake.