isolated synthetic tripeptide, isolated synthetic tripeptide conjugate, mycobacterium avium specific antigen, methods for in vitro detection of anti-map antibodies, diagnosing map infection, in vitro detection of humoral immune response, assessing cellular immune response in vitro, vaccination , genetically characterize a tested bacterium, genetically discriminate, detect presence of map cepa s, compositions, diagnostic kits, pentapeptide conjugate and antigenic composition

摘要

The present invention is directed to an isolated synthetic tripeptide of formula hd-phe-n-methyl-1-val-1-ala-ome (sequence id: 1), or a derivative thereof, and the corresponding lipotripeptides, which are: specific for mycobacterium avium subsp. paratuberculosis (map) of the s-type strain, as well as derivatives and conjugates thereof. The invention also relates to the use of these antigens in different methods and tests to detect map infection, especially by detecting humoral response and cell-mediated response of infected animals. The invention is also directed to a gene map signature and a characteristic signature obtained by mass spectrometry and nmr spectroscopy of the presence of map or map infection.