METHOD TO PRODUCE KIDNEY PROGENITOR CELLS, KIDNEY PROGENITER CELL, KIDNEY ORGANOID, PHARMACEUTICAL COMPOSITION, AND THERAPEUTIC AGENT FOR KIDNEY DISEASE.

摘要

a method for producing renal progenitor cells from pluripotent stem cells, comprising steps (i) to (vi): (i) cultivating pluripotent stem cells in a culture medium containing fgf2, bmp4, a gsk-3ß inhibitor and retinoic acid or a derivative thereof; (ii) culturing cells produced in step (i) in a culture medium containing fgf2, an inhibitor of gsk-3ß and bmp7; (iii) culturing cells produced in step (ii) in a medium containing fgf2, a gsk-3ß inhibitor, bmp7 and a tgfß inhibitor; (iv) cultivating cells produced in step (iii) in a culture medium containing fgf2, a gsk-3ß inhibitor, bmp7, activin and a rock inhibitor (rho-kinase); (v) culturing cells produced in step (iv) in a culture medium containing retinoic acid or a derivative thereof, and fgf9; and (vi) culturing cells produced in step (v) in a culture medium containing a gsk-3ß and fgf9 inhibitor, to induce renal progenitor cells from intermediate mesodermal cells.