HOUSING FUNGUS CEPA AND METHODS FOR HOMOLOGICAL AND / OR HETEROLOGIST PRODUCTION OF A PURE PROTEIN WITH A PURITY OF MORE THAN 75, FOR THE PRODUCTION OF ARTIFICIAL MIXTURES OF PROTEINS AND FOR SIMPLIFIED SCREENING OF CEPAS THAT EXPRESSLY EXCEED 5 ENOUGH ONLY ONE ENZYME

摘要

new fungus production system. the present invention provides a new fungal production system comprising a strain of host fungi of chucksosporium iucknowense where the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion deb chrysosporium lucknowense strain 18-25 uv. preferably, the secretion of endogenous protease, endogenous b-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of chrysosporium lucknowense strain 18-25 uv. in addition, host fungal strains are provided where several genes have been disrupted. according to another aspect of the invention a method for homologous and / or heterologous production of a pure protein with a purity greater than 75%, which comprises expressing a gene encoding said protein in a strain according to the invention has been described. in addition, a method for the production of artificial protein mixtures comprising the expression of genes encoding each of said proteins in a strain according to the invention has been disclosed. finally, a method for simplified screening of strains that functionally express an enzyme desired by the application of said strains was provided.